Gram Panchayat Haminpur Pilani Jhunjhunu


Gram Panchayat

Biology NET Questions with Answer

1. The inputs to one cycle of the urea cycle are

(A). 1 molecule of aspartic acid, 1 molecule of ammonia, 1 molecule of carbon dioxide, 3 molecules of ATP
(B). 1 molecule of urea, 1 molecule of ammonia, 3 molecules of ATP and 1 molecule of fumaric acid
(C). 1 molecule of fumaric acid, 1 molecule of urea, 3 molecules of AMP
(D). None of the above  

Correct Answer: A

2. Recognition/binding site of RNA polymerase is called

(A). receptor
(B). promoter
(C). facilitator
(D). terminator

Correct Answer: B

3. Soft pickles are made so by pectolytic enzymes mostly from

(A). moulds
(B). yeasts
(C). bacteria
(D). none of these

Correct Answer: A

4. Carrot is the main source of Vitamin


(A). A

(B). B
(C). C
(D). D

Correct Answer: A

5. The PCR, polymerase chain reaction is becoming the method of choice for

(A). Alteration of gene
(B). screening gene
(C). sterilization of gene
(D). all of these

Correct Answer: B

6. The bacteria deficient in cell wall is

(A). Treponema
(B). Mycoplasma
(C). Staphylococcus
(D). Klebsiella

Correct Answer: B

7. Which have the maximum resistance while transferring carbon dioxide from the bulk liquid to an air bubble?

(A). Moving through the gas liquid interface
(B). Moving through the bubble boundary layer
(C). Moving across the cell membrane
(D). Moving through the bulk liquid

Correct Answer: B

8. In lysogeny,

(A). a bacteriophage transfers bacterial DNA
(B). bacteria take up double stranded DNA from the environment
(C). DNA-degrading enzymes in the extracellular medium would stop the process
(D). a bacteriophage genome is integrated into the bacterial genome

Correct Answer: D

9. The major element of secondary structure in myoglobin and hemoglobin is

(A). the P-strand
(B). the a-helix
(C). the reverse turn
(D). All of these

Correct Answer: B

10. When metal is heated the density of metal

(A). increases
(B). first increases then decreases
(C). decreases
(D). remains the same

Correct Answer: C

11. Which one of the following is the link between Annelida and Mollusca ?

(A). Archaeopteryx 

(B). Peripatus

(C). Neopilina 

(D). Coelacanth

Correct Answer: C

12. During aeration, a cylindrical reactor had a height of 10 m. Without aeration, the height was 7.5 m. Under these aeration and mixing conditions, the gas hold up in the reactor is

(A). 100%
(B). 75%
(C). 50%
(D). 25%

Correct Answer: D

13. The maximum energy per gram on oxidization is yielded from

(A). fat
(B). protein
(C). glycogen
(D). starch

Correct Answer: A

14. Which of the following best explains the reasons for using axial flow impellers in crystallization and precipitation reactions?

(A). Crystal and precipitates are not too small, so as to ensure easy separation of the solids from the mother liquor
(B). Crystals and precipitates are thoroughly broken down, so as to ensure easy separation of the solids from the mother liquor
(C). Both (a) and (b)
(D). Axial flow impellers provide low shear, energy efficient mixing conditions

Correct Answer: A

15. Why phosphorescence measurements are usually made at a low temperature?

(A). To prevent thermal degradation of the phosphorescent species
(B). To promote phosphorescence by slowing the rate of radiationless transfer processes
(C). To increase the efficiency of the detector
(D). To decease the efficiency of detector

Correct Answer: B

16. Prokaryotic cells lack

(A). nucleolus
(B). nuclear membrane
(C). membrane bound by organelles
(D). All of these

Correct Answer: D

17. Which of the following best explains about the usefulness of the production of ethanol in yeast cells under anaerobic conditions?

(A). Ethanol keeps the electron transport system functioning
(B). Yeast would be unable to activate the enzymes of the Krebs cycle without ethanol
(C). The process generates oxygen, which is required for glycolysis
(D). The process regenerates NAD+, which is required for glycolysis

Correct Answer: D

18. Which of the following condition is non-suppurative sequelae of Streptococcus pyogenes infections?

(A). Acute rheumatic fever
(B). Acute glomerulonephritis
(C). Erythema nodosum
(D). All of these

Correct Answer: D

19. For brucellosis, 2 ME agglutination test is used to identify

(A). IgG
(B). IgM
(C). IgA
(D). IgE

Correct Answer: A

20. Which of the following is/are not a gram-positive bacteria?

(A). Streptococci
(B). Pseudomonas
(C). Mycobacteria
(D). None of these

Correct Answer: B

21. The biotin level in obtaining L-gluatmic acid by fermentation using Micrococcus glutamicus is critical because

(A). too low level prevents growths, hence L-glutamic acid production
(B). too high level prevents L-glutamic acid production
(C). both (a) and (b)
(D). none of the above

Correct Answer: C

22. Concentrate of fruits and vegetable juices

(A). favor the growth of A. niger and A. flavus species
(B). favour the growth of yeast and of acid and sugar tolerant Leuconostoc and Lactobacillus species
(C). favor the growth of Saprophytic bacteria
(D). none of the above

Correct Answer: B

23. What is the result of an abnormal response of the immune system to part of a person's own body?

(A). Passive immunity
(B). Cancer
(C). An allergic response
(D). An autoimmune disease

Correct Answer: D

24. If the stirrer speed is too slow, bubbles will accumulate and coalescese under the impeller. This phenomenon is known as a

(A). coalesced impeller
(B). flooded impeller
(C). river impeller
(D). dry impeller

Correct Answer: B

25. Vitamin-D deficiency can cause

(A). rickets
(B). pernicious anemia
(C). cataract
(D). beri-beri

Correct Answer: A

26. Sexual dimorphism is found in—

(A). Hydra 

(B). Earthworm

(C). Ascaris 

(D). Fasciola

Correct Answer: B

27. Canned poultry is more often spoiled by

(A). putrefactive than by Saccharolytic Clostridia
(B). Saccharolytic Clostridia than by putrefactive
(C). Saccharomyces species
(D). Micrococcus species

Correct Answer: A

28. The amount of CSF in the cranial cavity

(A). 500 ml
(B). 140 ml
(C). 1 litre
(D). 1.5 ml

Correct Answer: B

29. The bond between two amino acids in a protein is called

(A). Easter bond
(B). Peptide bond
(C). Glycosid bond
(D). phosphodiester bond

Correct Answer: A

30. Since ΔG° = -RTlnK

(A). a 10-fold increase in K decreases ΔG° by about 10-fold
(B). a 10-fold decrease in K decreases ΔG° by about 2.3RT
(C). a 10-fold increase in K decreases ΔG° by about 2.3RT
(D). a 10-fold decrease in K increases ΔG° by about 10-fold

Correct Answer: B

31. During glycolysis, the major energy generating step involves

(A). pyruvate kinase
(B). phosphoglycerate kinase
(C). glyceraldehyde-3 -dehydrogenase
(D). Phosphofructokinase

Correct Answer: C

32. The Redfield ratio is an index of concentration of

(A). carbon, hydrogen and oxygen
(B). nitrogen, potassium and iron
(C). carbon, nitrogen and phosphorous
(D). carbon, phosphorous and sulfur

Correct Answer: C

33. What is the term used for a segment of DNA with one or more genes in the centre and the two ends carrying inverted repeat sequences of nucleotides?

(A). Plasmid
(B). Transposon
(C). Insertion sequence
(D). None of these

Correct Answer: B

34. A disease producing species occurring in a mixed culture can often be selected on the basis of its

(A). pathogenic properties
(B). special carbon source
(C). special nitrogen source
(D). none of these

Correct Answer: A

35. The production of sufficient acid by fermentation of glucose leads to decrease in pH such that pH of the medium falls below 4.5. Which of the following test can detect it?

(A). Indole test
(B). Methyl red test
(C). Citrate utilization test
(D). Voges-Proskauer test

Correct Answer: B

36. Which of the following modified amino acid is used at the starting of most prokaryotic proteins?

(A). N-formylserine
(B). N-formylmethionine
(C). N-formylleucine
(D). N-formylalanine

Correct Answer: B

37. Which one of the following is produced in the greatest numbers during one turn of the Krebs cycle?

(B). Acetyl-CoA
(C). FADH2
(D). ATP

Correct Answer: A

38. Incorporation of atmospheric N2to NH4+ occurs via the process of

(A). assimilatory nitrate reduction
(B). transamination
(C). deamination
(D). nitrogen fixation

Correct Answer: D

39. The reactions of the cell that are carried out for capturing energy are called

(A). catabolism
(B). metabolism
(C). anabolism
(D). activation energy

Correct Answer: A

40. Which of the following is the most primitive?

(A). Virus
(B). Eukaryote
(C). Archaeon
(D). Mitochondria

Correct Answer: C

41. Pseudomonas aeruginosa forms a blue water soluble pigment called

(A). pyocyanin
(B). chlororaphin
(C). pyoverdin
(D). β-carotene

Correct Answer: A

42. The development of global regulatory systems in prokaryotes

(A). determines the exact location of the cell on the globe
(B). aids in DNA replication
(C). determines the exact location of the replication site
(D). regulates many genes under varying environmental conditions

Correct Answer: D

43. How do you differentiate a butterfly from a moth?

(A). Moth has one pair of wings but butterfly has two pairs of wings.
(B). Moth has feathery antennae but butterfly has club shaped antennae.
(C). Moth has simple eyes but butterfly has compound eyes.
(D). Moth is diurnal but butterfly is nocturnal.

Correct Answer: B

44. The fresh water sponge is—

(A). Spongella 

(B). Grantia

(C). Euplectella

(D). Euspongia

Correct Answer: A

45. Southern blotting involves

(A). electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
(B). electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
(C). electrophoresis of DNA molecules followed by incubation
(D). electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation

Correct Answer: A

46. The cause of cretinism is

(A). Hypothyroidism
(B). Hypoparathyroidism
(C). Hyperthyroidism
(D). Hyperparathyroidism

Correct Answer: A

47. Most transcripts of protein coding genes are cleaved

(A). at the 3' end just after a poly adenylation signal
(B). at the 3' end just before a poly adenylation signal
(C). at the 5' end immediately after a poly adenylation signal
(D). at the 5' end immediately before a poly adenylation signal

Correct Answer: A

48. Centriole is found in—

(A). Animal cells 

(B). Red algae

(C). Prokaryotes 

(D). Flowering plants

Correct Answer: A

49. By which of the following methods, new and better varieties of plants can be formed?

(A). Selection
(B). Grafting
(C). Hybridization
(D). Hybridization followed by selection

Correct Answer: D

50. Photosynthesis generally takes place in which parts of the plant?

(A). Leaf and other chloroplast bearing parts
(B). steam and leaf
(C). Roots and chloroplast bearing parts
(D). Bark and leaf

Correct Answer: A

51. Birbal Sahni Institute of Palaeobotany is situated at—

(A). Allahabad 

(B). Delhi

(C). Mumbai 

(D). Lucknow

Correct Answer: D

52. Gram positive cells have a

(A). second outer membrane that helps to retain the crystal violet stain
(B). multiple layer of peptidoglycan that helps to retain the crystal violet stain
(C). thick capsule that traps the crystal violet stain
(D). periplasmic space that traps the crystal violet

Correct Answer: B

53. Palak leaves are rich source of—

(A). Vitamin A

(B). Iron

(C). Carotene

(D). Vitamin E

Correct Answer: B

54. Nymph is the name of young one of

(A). butterfly
(B). beetle
(C). housefly
(D). cockroach

Correct Answer: D

55. The term, ‘southern blotting’ refers to

(A). transfer of DNA fragments from invitro cellulose membrane to electrophoresis gel

(B). attachment of probes to DNA fragments

(C). transfer of DNA fragments from electrophoresis gel to nitrocellulose sheet.

(D). comparison of DNA fragments from two sources


Correct Answer: C

56. The "A" subunit of diphtheria toxin

(A). binds host cell receptors found on heart cells
(B). cause ADP ribosylation of a factor involved in protein synthesis
(C). forms cAMP that leads to fluid accumulation
(D). lysis macrophages with the release of cytokines

Correct Answer: B

57. Which of the following relationships between absorbance and %transmittance is incorrect?

(A). A = log10 100 / %T
(B). A = 2 - log10 %T
(C). A = log10 1 / T
(D). All are correct

Correct Answer: C

58. The innate tendency of a material body to remain in a state in which it is placed originally is characterized qualitatively as ------ of the body.

(A). Rest
(B). Inertia
(C). Momentum
(D). Retardation

Correct Answer: C

59. Counter-immunoelectrophoresis is used for the detection of

(A). Meningococcal antigen
(B). Hepatitis B surface antigen
(C). Alpha-fetoprotein
(D). All of these

Correct Answer: D

60. The present day high yielding penicillin are obtained from

(A). Penicillium chrysogenum (NRRL, 1951)
(B). Penicillium chrysogenum (Q-176)
(C). Penicillium chrysogenum (Q-179)
(D). none of the above

Correct Answer: B

61. Each tRNA molecule has a cloverleaf secondary structure consisting of

(A). three stem loops
(B). two stem loops
(C). four stem loops
(D). either (a) or (b)

Correct Answer: A

62. When was the genetic code completed?

(A). 1958
(B). 1952
(C). 1966
(D). 1968

Correct Answer: C

63. The cell lines which can support the growth of polioviruses is/are

(A). Human amnion
(B). Monkey kidney
(C). HeLa
(D). All of these   

Correct Answer: D

64. For specific antigen recognition by T cells,

(A). antigen is bound by a T cell membrane antibody
(B). denaturation of antigen does not reduce epitope recognition
(C). MHC molecules are not required
(D). antigen exposure during T cell maturation is required

Correct Answer: B

65. The chromosomal genes, possessing fertility factor is known as

(A). R factor
(B). F prime factor
(C). HFr
(D). F factor

Correct Answer: B

66. In the three domain system of classification, the traditional bacteria are placed in the

(A). eukarya
(B). archaea
(C). eubacteria
(D). none of these

Correct Answer: C

67. The phenomenon of commensalism refers to a relationship between organisms in which

(A). one species of a pair benefits
(B). both the species of a pair benefit
(C). one species of a pair is more benefited
(D). none of the above

Correct Answer: A

68. Which of the following three codons translate as serine (Ser)?

(A). AGU
(B). CGA
(C). CAU
(D). AUG

Correct Answer: A

69. Mumps is a disease caused by

(A). fungus
(B). bacterium
(C). virus
(D). None of these

Correct Answer: C

70. The last step in synthesis of peptidoglycan is

(A). attachment of a peptide to muramic acid
(B). attaching two amino acids to form a cross-link
(C). attachment of a portion of peptidoglycan to a membrane lipid
(D). binding of penicillin to a membrane protein

Correct Answer: B

71. Which of the following statements is incorrect?

(A). Protein G contains both a-helix and P-sheet
(B). Protein G contains only a-helix
(C). Fatty acid binding protein contains largely P-sheet
(D). Hemoglobin contains four sub-units

Correct Answer: B

72. Supercoiling refers to the extra turns in the structure of ds DNA due to the action of

(A). DNA gyrase
(B). DNA pyrase
(C). RNA gyrase
(D). RNA pyrase

Correct Answer: A

73. Brucella are

(A). Cocci
(B). Rods
(C). Cocobacilli
(D). Very short rods   

Correct Answer: D

74. The cells most oftenly infected by HIV are

(A). Null cells
(B). CD8 + T lymphocytes
(C). CD4 + T lymphocytes
(D). None of these

Correct Answer: C

75. An oil drop with a polar coat is a metaphor referring to the three dimensional structure of

(A). fibrous proteins
(B). collagen
(C). globular proteins
(D). silk protein

Correct Answer: C

76. In cellular metabolism, O2 is used

(A). to provide electrons for photophosphorylation
(B). in glycolysis
(C). as a terminal electron acceptor
(D). in the Krebs cycle

Correct Answer: C

77. Which one is product of aerobic respiration?

(A). Malic acid
(B). Ethyl alcohol
(C). Lactic acid
(D). Pyruvic acid

Correct Answer: A

78. Lung fish is a link between


(A). Amphibia and Birds
(B). Reptiles and Birds

(C). Amphibia and Reptiles
(D). Reptiles and Mammals

Correct Answer: C

79. Clostridium botulinum food poisoning is due to

(A). invasion of bacteria in the intestine
(B). preformed toxin
(C). both (a) and (b)
(D). none of the above

Correct Answer: B

80. The commonest opportunistic infection in AIDS patient in India is

(A). cryptosporidiosis
(B). toxoplasmosis
(C). tuberculosis
(D). cryptococcosis

Correct Answer: C

81. The ability of CTP to bind to aspartate carbamoyltransferase and shut down the synthesis of more CTP is an example of

(A). enzyme induction
(B). enzyme repression
(C). feedback inhibition of enzyme activity
(D). none of these

Correct Answer: C

82. The peptide bond in proteins is

(A). planar, but rotates to three preferred dihedral angles
(B). nonpolar, but rotates to three preferred dihedral angles
(C). nonpolar, and fixed in a trans conformation
(D). planar, and usually found in a trans conformation

Correct Answer: D

83. Which are phagocytes in liver ?

(A). Kupffer cells 

(B). Aciner cells

(C). Diter cells 

(D). Hensen's cells

Correct Answer: A

84. Sexual reproduction in the Protozoa occurs most commonly by

(A). conjugation
(B). gametangial contact
(C). binary fission
(D). binary fusion

Correct Answer: A

85. In the mammals, cleavage result formation of solid mass of cells known-

(A). Blastula 

(B). Gastrula

(C). Morula 

(D). Neurula

Correct Answer: C

86. A cell develops the same power across two resistance R1 and R2 separately. The internal resistance of the cell is

(A). R1 + R2
(B). R1 + R2 /2
(C). √R1R2
(D). √R1R2 /2

Correct Answer: C

87. The damaged ozone layer is situated in-

(A). Ionosphere 

(B). Mesosphere

(C). Stratosphere 

(D). Troposphere

Correct Answer: C

88. The organism commercially used for fungal fermentation to obtain citric acid is

(A). A. oryzae
(B). A.flavus
(C). A. niger
(D). S. cerevisiae

Correct Answer: C

89. Kidneys are vital organs of the body because they help in—

(A). Regulation of body fluids

(B). Regulation of acid-base balance

(C). Removal of metabolic wastes

(D). All of these functions

Correct Answer: D

90. Which of the following blood group is universal acceptor ?

(A). A
(B). B
(C). AB

(D). O

Correct Answer: C

91. Fleshy fruits with stony endocarp are called

(A). .Berries
(B). .Pomes
(C). .Drupes
(D). .Capsules

Correct Answer: C

92. Which f the following is an example of man-made ecosystem?

(A). Herbarium
(B). Aquarium
(C). Tissue culture
(D). Forest

Correct Answer: B

93. Number of cotyledons found in the embryo of Cycas is—

(A). 4 

(B). 2

(C). 3 

(D). 1

Correct Answer: B

94. Hemoglobin containing iron is—

(A). Antibody
(B). Nucleic Acid
(C). Protein
(D). Hormone

Correct Answer: C

95. T-even phage binding to E. coli probably involves

(A). electrostatic interaction
(B). hydrophobic interaction
(C). covalent bonds
(D). all of these

Correct Answer: A

96. Spermatids derive the nourishment from

(A). nucleus
(B). ciliated Epithelial
(C). cytoplasm
(D). sartoli cell

Correct Answer: B

97. Long-chain fatty acids are oxidized step-wise in one carbon units starting from the

(A). carboxyl end
(B). aliphatic end
(C). both (a) and (b)
(D). none of these

Correct Answer: A

98. The third kingdom, protista, as suggested by E.H. Haeckel includes

(A). bacteria
(B). algae
(C). fungi
(D). all of these

Correct Answer: D

99. Which of the following antibiotics can't be used for treatment of infections with β-lactamase-producing Staphylococcus aureus?

(A). Vancomycin
(B). Ampicillin/sulbactam
(C). Cephalothin
(D). Piperacillin

Correct Answer: D

100. Pinus belongs to the class ________.

(A). Gentopsida
(B). Cycadopsida
(C). Coniferopsida
(D). Sphenopsida

Correct Answer: C

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